ZBTB20 Polyklonaler Antikörper

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ZBTB20 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23987-1-PBS

Synonyme

Dendritic-derived BTB/POZ zinc finger protein, DPZF, Zinc finger and BTB domain-containing protein 20, Zinc finger protein 288, ZNF288

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  WB analysis using 23987-1-AP (same clone as 23987-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 23987-1-AP (same clone as 23987-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 23987-1-AP (same clone as 23987-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 23987-1-AP (same clone as 23987-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 23987-1-AP (same clone as 23987-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 23987-1-AP (same clone as 23987-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 23987-1-AP (same clone as 23987-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 23987-1-AP (same clone as 23987-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 23987-1-AP (same clone as 23987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23987-1-AP (ZBTB20 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 23987-1-AP (same clone as 23987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23987-1-AP (ZBTB20 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 23987-1-AP (same clone as 23987-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ZBTB20 antibody (23987-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 23987-1-AP (same clone as 23987-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using ZBTB20 antibody (23987-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse liver using 23987-1-AP (same clone as 23987-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using ZBTB20 antibody (23987-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

23987-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA ZBTB20 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZBTB20 fusion protein Ag21140
• Vollständiger Name: zinc finger and BTB domain containing 20
• Berechnetes Molekulargewicht: 668 aa, 73 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC029041
• Gene symbol: ZBTB20
• Gene ID (NCBI): 26137
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZBTB20 (zinc finger and BTB domain containing 20), also known as HOF, DPZF, ODA-8S or ZNF288, is a 741 amino acid protein that localizes to the nucleus and contains one BTB (POZ) domain and five C2H2-type zinc fingers. Expressed in thymus, spleen, lymph node and fetal liver, ZBTB20 exists as either a monomer or a homodimer that is thought to function as a transcription factor, playing a role in hematopoiesis, oncogenesis and immune responses. Multiple isoforms of ZBTB20 exist due to alternative splicing events.