ZBTB33 Polyklonaler Antikörper

ZBTB33 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55270-1-PBS

Synonyme

ZNF348, ZNF kaiso, Zinc finger and BTB domain-containing protein 33, Transcriptional regulator Kaiso, KAISO

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  WB analysis of A431 using 55270-1-AP (same clone as 55270-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 55270-1-AP (ZBTB33 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55270-1-PBS in a different storage buffer formulation.

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  IP experiment of A431 using 55270-1-AP (same clone as 55270-1-PBS)

  IP result of anti-ZBTB33 (IP:55270-1-AP, 4ug; Detection:55270-1-AP 1:300) with A431 cells lysate 1200ug. This data was developed using the same antibody clone with 55270-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 55270-1-AP (same clone as 55270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 55270-1-AP (ZBTB33 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55270-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 55270-1-AP (same clone as 55270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 55270-1-AP (ZBTB33 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55270-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

55270-1-PBS bindet in WB, IHC, IP, Indirect ELISA ZBTB33 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: zinc finger and BTB domain containing 33
• Berechnetes Molekulargewicht: 74 kDa
• Beobachtetes Molekulargewicht: 68-74 kDa
• GenBank-Zugangsnummer: NM_006777
• Gene symbol: ZBTB33
• Gene ID (NCBI): 10009
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZBTB33, also named as Transcriptional regulator Kaiso, is a 672 amino acid protein, which localizes in nucleus and cytoplasm. ZBTB33 as a transcriptional regulator with bimodal DNA-binding specificity binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). ZBTB33 recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters and may contribute to the repression of target genes of the Wnt signaling pathway. The calculated molecular weight of ZBTB33 is 75 kDa, but modified protein is about 100 kDa (PMID: 10207085 and PMID: 16354691)