ZBTB7B Polyklonaler Antikörper

ZBTB7B Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 11341-1-AP

Synonyme

c Krox, hcKrox, THPOK, ZBTB15, ZBTB7B, Zfp 67, ZFP67, Zinc finger protein 67 homolog, Zinc finger protein Th POK, ZNF857B

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Galerie der Validierungsdaten

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  WB analysis using 11341-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11341-1-AP (ZBTB7B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11341-1-AP

  IP result of anti-ZBTB7B(IP:11341-1-AP, 4ug; Detection:11341-1-AP 1:3000) with HeLa cells lysate 1320 ug.

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  IF Staining of HeLa using 11341-1-AP

  Immunofluorescent analysis of Hela cells, using ZBTB7B antibody 11341-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of MCF-7 using 11341-1-AP

  Immunofluorescent analysis of HepG2 cells, using ZBTB7B antibody 11341-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

11341-1-AP bindet in WB, IP, IHC, IF, ELISA ZBTB7B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZBTB7B fusion protein Ag1888
• Vollständiger Name: zinc finger and BTB domain containing 7B
• Berechnetes Molekulargewicht: 539 aa, 58 kDa
• Beobachtetes Molekulargewicht: 60-65 kDa
• GenBank-Zugangsnummer: BC012070
• Gene symbol: ZBTB7B
• Gene ID (NCBI): 51043
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZBTB7B belongs to a large family of transcription factors, generally acting as repressors, characterized by a carboxy-terminal DNA binding domain made of multiple zinc fingers (four in Thpok) and an amino-terminal BTB-POZ domain that mediates homo- (and possibly hetero-) dimerization [PMID: 17084908]. Zbtb7b is up-regulated by MHC-II-restricted thymocytes during their CD4 differentiation and is a major determinant of CD4 lineage choice. Two properties of ZBTB7B deserve attention. First, although Thpok is expressed in a wide variety of cells, its expression in the thymus is highly lineage-specific : CD4 SP thymocytes (and all CD4 T cells) express Thpok, whereas DP and CD8 SP thymocytes do notSecond, both loss- and gain-of-function experiments indicate that Thpok affects lineage choice but not positive selection [PMID: 15729333, 10550051].

Publikationen

Species	Application	Title
hamster	WB	Acta Biomater; LAP2β transmits force to upregulate genes via chromatin domain stretching but not compression; Authors - Jian Sun
human	WB,IHC	J Transl Med; Epigenetic DNA methylation of Zbtb7b regulates the population of double-positive CD4+CD8+ T cells in ulcerative colitis.; Authors - Hao-Ming Xu
bovine	WB,IP	J Agric Food Chem; Acylated Ghrelin Activates PI3K/mTOR Signaling Pathway by Promoting ThPOK Acetylation to Promote Milk Fat Synthesis in Bovine Mammary Epithelial Cells; Authors - Jiaxin Wang