ZC3H12D Polyklonaler Antikörper

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• KD/KO Validated

ZC3H12D Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 24991-1-AP

Synonyme

C6orf95, MCP induced protein 4, MCPIP4, p34, Probable ribonuclease ZC3H12D, TFL, ZC3H12D

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Galerie der Validierungsdaten

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  WB analysis of Raji using 24991-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 24991-1-AP (ZC3H12D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 24991-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24991-1-AP (ZC3H12D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 24991-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 24991-1-AP (ZC3H12D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 24991-1-AP

  IP Result of anti-ZC3H12D (IP:24991-1-AP, 4ug; Detection:24991-1-AP 1:600) with Raji cells lysate 2000ug.

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  IHC staining of human tonsillitis using 24991-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24991-1-AP (ZC3H12D Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 24991-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24991-1-AP (ZC3H12D Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, K-562-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Produktinformation

24991-1-AP bindet in WB, IP, IHC, ELISA ZC3H12D und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZC3H12D fusion protein Ag21770
• Vollständiger Name: zinc finger CCCH-type containing 12D
• Berechnetes Molekulargewicht: 527 aa, 58 kDa
• Beobachtetes Molekulargewicht: 55-58 kDa
• GenBank-Zugangsnummer: BC157832
• Gene symbol: ZC3H12D
• Gene ID (NCBI): 340152
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZC3H12D, also named as MCP induced protein 4, is a 527 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12D exists as three isoforms and localizes in the cytoplasm. ZC3H12D may regulate cell growth likely by suppressing RB1 phosphorylation and serves as a tumor suppressor in certain leukemia cells. ZC3H12D is expressed in normal human lymphocytes but defective in some leukemia/lymphoma cell lines.

Publikationen

Species	Application	Title
human	IHC	Cell Rep Med; Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application; Authors - Xiaoxue Ren
	WB	Nat Commun; Extracellular mRNA transported to the nucleus exerts translation-independent function.; Authors - Takeshi Tomita ; KO Validated
human	WB	Comput Struct Biotechnol J; Integrating knowledge of protein sequence with protein function for the prediction and validation of new MALT1 substrates; Authors - Peter A Bell
mouse	WB	J Biol Chem; Monocyte Chemotactic Protein-induced Protein 1 and 4 Form a Complex but Act Independently in Regulation of Interleukin-6 mRNA Degradation.; Authors - Shengping Huang ; KD Validated
human	WB	Front Aging Neurosci; DNA Methylation Profiling Reveals the Change of Inflammation-Associated ZC3H12D in Leukoaraiosis.; Authors - Wen-Qing Huang
human	WB	Oncol Lett; miR-128-3p serves as an oncogenic microRNA in osteosarcoma cells by downregulating ZC3H12D.; Authors - Maoshu Zhu