ZC3H15 Polyklonaler Antikörper

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• KD/KO Validated

ZC3H15 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26241-1-AP

Synonyme

DFRP1, HT010, LEREPO4, MSTP012, PP730, ZC3H15

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  WB analysis of K-562 using 26241-1-AP

  WB result of ZC3H15 antibody (26241-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZC3H15 transfected K-562 cells.

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  WB analysis of HeLa using 26241-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26241-1-AP (ZC3H15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 26241-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 26241-1-AP (ZC3H15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 26241-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26241-1-AP (ZC3H15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 26241-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26241-1-AP (ZC3H15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 26241-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26241-1-AP (ZC3H15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 26241-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using ZC3H15 antibody (26241-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 26241-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26241-1-AP (ZC3H15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HL-60-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26241-1-AP bindet in WB, IHC, IF/ICC, ELISA ZC3H15 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZC3H15 fusion protein Ag24091
• Vollständiger Name: zinc finger CCCH-type containing 15
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC021102
• Gene symbol: ZC3H15
• Gene ID (NCBI): 55854
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.