ZC3H8 Monoklonaler Antikörper

ZC3H8 Monoklonal Antikörper für Indirect ELISA, WB

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

Indirect ELISA, WB

Konjugation

Unkonjugiert

CloneNo.

1G8C6

Kat-Nr. : 68282-1-PBS

Synonyme

Zinc finger CCCH domain-containing protein 8, ZC3HDC8, Fliz1, 1G8C6

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  WB analysis using 68282-1-Ig (same clone as 68282-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68282-1-Ig (ZC3H8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68282-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 68282-1-Ig (same clone as 68282-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68282-1-Ig (ZC3H8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68282-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 68282-1-Ig (same clone as 68282-1-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug ZC3H8 Monoclonal antibody (68282-1-Ig, Clone:1G8C6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 68282-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68282-1-PBS bindet in Indirect ELISA, WB ZC3H8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ZC3H8 fusion protein Ag23496
• Vollständiger Name: zinc finger CCCH-type containing 8
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC032001
• Gene symbol: ZC3H8
• Gene ID (NCBI): 84524
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZC3H8, also named as Fliz1, is a novel zinc finger protein preferentially expressed in fetal liver hematopoietic progenitors and in several adult organs, including the thymus. It acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene. Overexpression of Fliz1 repressed the expression of GATA-3 in vivo and in vitro. GATA-3 might be partly regulated by the intronic regulatory region and Fliz1 in a developmental stage-specific fashion (PMID: 23932780).