ZC3HAV1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

ZC3HAV1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

1G10B9

Kat-Nr. : 66413-1-Ig

Synonyme

FLB6421, ZAP, ZC3H2, ZC3HAV1, ZC3HDC2, Zinc finger antiviral protein

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 66413-1-Ig

  WB result of ZC3HAV1 antibody (66413-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZC3HAV1 transfected HEK-293 cells.

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  WB analysis of Jurkat using 66413-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66413-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66413-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 66413-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:300 (under 40x lens).

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  IHC staining of human kidney using 66413-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:300 (under 10x lens).

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  IF Staining of HepG2 using 66413-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66413-1-Ig(ZC3HAV1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

66413-1-Ig bindet in WB, IHC, IF, ELISA ZC3HAV1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ZC3HAV1 fusion protein Ag10364
• Vollständiger Name: zinc finger CCCH-type, antiviral 1
• Berechnetes Molekulargewicht: 699aa,78 kDa; 902aa,101 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC025308
• Gene symbol: ZC3HAV1
• Gene ID (NCBI): 56829
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

he zinc-finger antiviral protein (ZAP) was originally identifed from a rat cDNA library due to its conferring resistance to retroviruses. ZC3HAV1 is a CCCH-type zinc finger protein, it may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms.

Publikationen

Species	Application	Title
human	WB	mBio; The Zinc Finger Antiviral Protein ZAP Restricts Human Cytomegalovirus and Selectively Binds and Destabilizes Viral UL4/UL5 Transcripts.; Authors - Ana Cristina Gonzalez-Perez ; KO Validated
human	IF	J Virol; Riplet Binds the Zinc Finger Antiviral Protein (ZAP) and Augments ZAP-Mediated Restriction of HIV-1.; Authors - Marlene V Buckmaster
human	WB	EMBO Rep; Interferon inhibits a model RNA virus via a limited set of inducible effector genes; Authors - Matthew B McDougal