ZC3HAV1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

ZC3HAV1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G10B9

Kat-Nr. : 66413-1-PBS

Synonyme

PRO1677, PARP13, Inactive Poly [ADP-ribose] polymerase 13, ARTD13, ADP-ribosyltransferase diphtheria toxin-like 13

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  WB analysis of HEK-293 using 66413-1-Ig (same clone as 66413-1-PBS)

  WB result of ZC3HAV1 antibody (66413-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZC3HAV1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66413-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66413-1-Ig (same clone as 66413-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66413-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66413-1-Ig (same clone as 66413-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66413-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66413-1-Ig (same clone as 66413-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66413-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66413-1-Ig (same clone as 66413-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66413-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66413-1-Ig (same clone as 66413-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66413-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66413-1-Ig (same clone as 66413-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZC3HAV1 antibody (66413-1-Ig, Clone: 1G10B9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66413-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66413-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ZC3HAV1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ZC3HAV1 fusion protein Ag10364
• Vollständiger Name: zinc finger CCCH-type, antiviral 1
• Berechnetes Molekulargewicht: 699aa,78 kDa; 902aa,101 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC025308
• Gene symbol: ZC3HAV1
• Gene ID (NCBI): 56829
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

he zinc-finger antiviral protein (ZAP) was originally identifed from a rat cDNA library due to its conferring resistance to retroviruses. ZC3HAV1 is a CCCH-type zinc finger protein, it may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms.