ZCCHC11 Polyklonaler Antikörper

ZCCHC11 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 20119-1-AP

Synonyme

TUTase 4, TUT4, Terminal uridylyltransferase 4, PAPD3, KIAA0191

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  WB analysis of HeLa using 20119-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20119-1-AP (ZCCHC11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20119-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20119-1-AP (ZCCHC11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20119-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20119-1-AP (ZCCHC11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 20119-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20119-1-AP (ZCCHC11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 20119-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20119-1-AP (ZCCHC11 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 20119-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20119-1-AP (ZCCHC11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 20119-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20119-1-AP (ZCCHC11 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 20119-1-AP

  Immunofluorescent analysis of Hela cells, using ZCCHC11 antibody 20119-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

20119-1-AP bindet in WB, IHC, IF/ICC, ELISA ZCCHC11 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZCCHC11 fusion protein Ag13661
• Vollständiger Name: zinc finger, CCHC domain containing 11
• Berechnetes Molekulargewicht: 185 kDa
• Beobachtetes Molekulargewicht: 185 kDa
• GenBank-Zugangsnummer: BC048301
• Gene symbol: ZCCHC11
• Gene ID (NCBI): 23318
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZCCHC11, also named as KIAA0191 and TUT4, is an uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. ZCCHC11 catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. ZCCHC11 can't bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. ZCCHC11 may also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP.

Publikationen

Species	Application	Title
human	WB,IF	Neoplasia; Identification of novel targets of miR-622 in hepatocellular carcinoma reveals common regulation of cooperating genes and outlines the oncogenic role of zinc finger CCHC-type containing 11.; Authors - Anne Gaza