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  • KD/KO Validated

ZCCHC8 Polyklonaler Antikörper

ZCCHC8 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 23374-1-AP

Synonyme

ZCCHC8



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HepG2-Zellen, Maus-Thymusgewebe
Erfolgreiche Detektion in IHChumanes Hodengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:12000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

23374-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA ZCCHC8 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ZCCHC8 fusion protein Ag19985
Vollständiger Name zinc finger, CCHC domain containing 8
Berechnetes Molekulargewicht 707 aa, 79 kDa
Beobachtetes Molekulargewicht 90-100 kDa
GenBank-ZugangsnummerBC065918
Gene symbol ZCCHC8
Gene ID (NCBI) 55596
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The eukaryotic RNA exosome participates extensively in RNA processing and degradation. In human cells, three accessory factors (RBM7, ZCCHC8 and hMTR4) interact to form the nuclear exosome targeting (NEXT) complex, which directs a subset of non-coding RNAs for exosomal degradation. ZCCHC8 acts as a scaffold, mediating the interaction between RBM7 and MTR4. NEXT is involved in exosome-mediated surveillance and decay of noncoding RNAs, such as enhancer RNAs (eRNAs) and aberrant 3′-extended transcripts from small nuclear RNA (snRNA), telomerase RNA, and replication-dependent histone genes. NEXT is an important node in regulating nuclear exosome activities and takes part in several biological processes, including DNA damage response, stress response, and viral ribogenesis . ZCCHC8 also has a robust interaction with YTHDC1 (PMID: 28984244).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for ZCCHC8 antibody 23374-1-APProtokoll herunterladen
IHC protocol for ZCCHC8 antibody 23374-1-APProtokoll herunterladenl
IF protocol for ZCCHC8 antibody 23374-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB,IP

Mol Cell

Nuclear RNA catabolism controls endogenous retroviruses, gene expression asymmetry, and dedifferentiation

Authors - Denis Torre
  • KD Validated
humanWB

Nucleic Acids Res

mRNAs are sorted for export or degradation before passing through nuclear speckles.

Authors - Jing Fan
humanCoIP

Nucleic Acids Res

Concurrent binding to DNA and RNA facilitates the pluripotency reprogramming activity of Sox2.

Authors - Linlin Hou
mouseWB

Cell Rep

Nuclear Exosome Targeting Complex Core Factor Zcchc8 Regulates the Degradation of LINE1 RNA in Early Embryos and Embryonic Stem Cells.

Authors - You Wu
  • KO Validated
humanWB

Nat Neurosci

Globally reduced N6-methyladenosine (m6A) in C9ORF72-ALS/FTD dysregulates RNA metabolism and contributes to neurodegeneration

Authors - Yini Li
  • KD Validated
humanWB

PLoS Genet

RBMX involves in telomere stability maintenance by regulating TERRA expression

Authors - Jingfan Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Fabienne (Verified Customer) (05-09-2025)

Perfect for detecting ZCCHC8 by western blot. Tested on nuclear cell extracts

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Jurkat
ZCCHC8 Antibody Western Blot validation (1:1000 dilution) in Jurkat (Cat no:23374-1-AP)
FH

Tsimafei (Verified Customer) (04-22-2025)

Great antibody for Zcchc8 detection in mESCs

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mESCs
ZCCHC8 Antibody Western Blot validation (1:2000 dilution) in mESCs (Cat no:23374-1-AP)
FH

Moritz (Verified Customer) (08-20-2024)

I used this antibody to detect ZCCHC8 in HEK293T WT cell lysate on Western blot, which worked perfectly. The signal was very specific, no unspecific bands were detectable.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: HEK293T WT
FH

Roy (Verified Customer) (06-15-2024)

Perfect for detecting the NEXT complex component ZCCHC8 by WB (1h incubation at room temperature, 1/1000 dilution)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa