ZDHHC13 Polyklonaler Antikörper

ZDHHC13 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 24759-1-AP

Synonyme

Huntingtin-interacting protein 14-related protein, HIP3RP, HIP14-related protein, HIP14L, EC:2.3.1.225

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  WB analysis of mouse kidney using 24759-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 24759-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 24759-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 24759-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24759-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 24759-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 24759-1-AP (ZDHHC13 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 24759-1-AP

  Immunofluorescent analysis of HEK-293 cells using 24759-1-AP (ZDHHC13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HEK-293-Zellen, Maushautgewebe, Maushodengewebe, Neuro-2a-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

24759-1-AP bindet in WB, IHC, IF/ICC, ELISA ZDHHC13 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZDHHC13 fusion protein Ag21607
• Vollständiger Name: zinc finger, DHHC-type containing 13
• Berechnetes Molekulargewicht: 622 aa, 71 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC056152
• Gene symbol: ZDHHC13
• Gene ID (NCBI): 54503
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Palmitoyltransferase ZDHHC13 also named as HIP14L or DHHC13 is a 622 amino acid protein, which contains one DHHC-type zinc finger and six ANK repeats. ZDHHC13 localizes in the Golgi apparatus membrane and belongs to the DHHC palmitoyltransferase family. ZDHHC13 is a palmtoyltransferase for HD and GAD2, meanwhile it mediates Mg2+ transportation. ZDHHC13 exists as three alternative splicing isoforms. The molecular weight of isoform 2 is 51 kDa, but we often detected a 45-50kDa protein by western blot.

Publikationen

Species	Application	Title
human	IF, WB	J Cell Biol; Regulation of Golgi turnover by CALCOCO1-mediated selective autophagy.; Authors - Thaddaeus Mutugi Nthiga