ZDHHC5 Rekombinanter Antikörper

ZDHHC5 Rekombinant Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

242192A4

Kat-Nr. : 84803-4-RR

Synonyme

242192A4, DHHC-5, EC:2.3.1.225, KIAA1748, Palmitoyltransferase ZDHHC5

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  WB analysis using 84803-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84803-4-RR (ZDHHC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 84803-4-RR

  IP result of anti-ZDHHC5 (IP:84803-4-RR, 4ug; Detection:84803-4-RR 1:1000) with HEK-293 cells lysate 1720 ug.

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  IHC staining of human ovary cancer using 84803-4-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84803-4-RR (ZDHHC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 84803-4-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84803-4-RR (ZDHHC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84803-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84803-4-RR against Human ZDHHC5 were performed. The affinity constant is 2.16 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A549-Zellen, Maushirngewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84803-4-RR bindet in WB, IHC, IP, ELISA ZDHHC5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ZDHHC5 fusion protein Ag15022
• Vollständiger Name: zinc finger, DHHC-type containing 5
• Berechnetes Molekulargewicht: 715 aa, 78 kDa
• Beobachtetes Molekulargewicht: 75-77 kDa
• GenBank-Zugangsnummer: BC026967
• Gene symbol: ZDHHC5
• Gene ID (NCBI): 25921
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The gene encoding zinc finger DHHC-type-containing (ZDHHC)5 is located in a region of chromosome 11q12.1 associated with a high rate of chromosomal translocation and variation, which has been linked to the development of various cancers. ZDHHC5 stimulates the proliferation and anchorage-dependent and -independent colony formation of non-small cell lung cancer cell lines, and was found to be required in a subset of these cells for establishment of tumor xenografts in mice. ZDHHC5 is highly expressed in a variety of embryonic cells including neural progenitor cells, but not in most adult tissues.

Publikationen

Species	Application	Title
		J Virol; Palmitoylation enhances the stability of porcine epidemic diarrhea virus spike protein by antagonizing its degradation via chaperone-mediated autophagy to facilitate viral proliferation; Authors - Qisheng Qian