ZDHHC9 Polyklonaler Antikörper

ZDHHC9 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 24046-1-AP

Synonyme

EC:2.3.1.225, DHHC-9, DHHC9, DHHC 9, CXorf11

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  WB analysis using 24046-1-AP

  Mouse liver tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 24046-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 24046-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 24046-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 24046-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 24046-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 24046-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 24046-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24046-1-AP (ZDHHC9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 24046-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of SH-SY5Y using 24046-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 24046-1-AP (ZDHHC9 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Maushirngewebe, Maus-Skelettmuskelgewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

24046-1-AP bindet in WB, IHC, IF/ICC, ELISA ZDHHC9 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZDHHC9 fusion protein Ag20881
• Vollständiger Name: zinc finger, DHHC-type containing 9
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 38-48 kDa
• GenBank-Zugangsnummer: BC006200
• Gene symbol: ZDHHC9
• Gene ID (NCBI): 51114
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZDHHC9, also named as CXorf11, ZDHHC10, ZNF379 or ZNF380, is a 364 amino acid protein, which contains one DHHC-type zinc finger and belongs to the DHHC palmitoyltransferase activity. ZDHHC9 localizes in the endoplasmic reticulum membrane and is highly expressed in kidney, skeletal muscle, brain, lung and liver. The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.

Publikationen

Species	Application	Title
human,mouse	IHC,IF	Discov Oncol; ZDHHC9: a promising therapeutic target for triple-negative breast cancer through immune modulation and immune checkpoint blockade resistance; Authors - Chao Xu
human	WB	Cancer Lett; Palmitoylation alters LDHA activity and pancreatic cancer response to chemotherapy; Authors - Luojun Chen
mouse	WB,IF	Adv Mater; Multifunctional Copper-Phenolic Nanopills Achieve Comprehensive Polyamines Depletion to Provoke Enhanced Pyroptosis and Cuproptosis for Cancer Immunotherapy; Authors - Guoqing Zhu