• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ZFC3H1 Polyklonaler Antikörper

ZFC3H1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30834-1-AP

Synonyme

CCDC131, CSRC2, KIAA0546, PSRC2

Filter:
  • Western Blot (WB) analysis of various lysates using ZFC3H1 Polyclonal antibody (30834-1-AP)

    WB analysis using 30834-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30834-1-AP (ZFC3H1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ZFC3H1 Polyclonal antibody (30834-1-AP)

    IF Staining of U2OS using 30834-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZFC3H1 antibody (30834-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBDU 145-Zellen, HEK-293T-Zellen
Erfolgreiche Detektion in IF/ICCU2OS-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

30834-1-AP bindet in WB, IF/ICC, ELISA ZFC3H1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ZFC3H1 fusion protein Ag34224
Vollständiger Name zinc finger, C3H1-type containing
Berechnetes Molekulargewicht226 kDa
Beobachtetes Molekulargewicht250 kDa
GenBank-ZugangsnummerBC156732
Gene symbol ZFC3H1
Gene ID (NCBI) 196441
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZFC3H1, also known as CCDC131 and PSRC2, is a large protein (1989 amino acids), with a C3H1- type zinc finger motif in its center, and contains five tetratricopeptide (TRP) repeats and six halftetratricopeptide (HAT) repeats at the C-terminal region. It is involved in the processing of a variety of RNAs and plays a critical role in the degradation of nuclear RNAs (PMID: 34514952).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for ZFC3H1 antibody 30834-1-APProtokoll herunterladen
IF protocol for ZFC3H1 antibody 30834-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Fabienne (Verified Customer) (09-12-2025)

Works great in immunoprecipitation (used 3µg antibody per 250µg nuclear extracts)

  • Applications: Immunoprecipitation
  • Cell Tissue Type: Jurkat