ZFP161 / ZBTB14 Rekombinanter Antikörper

ZFP161 / ZBTB14 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240853B12

Kat-Nr. : 83909-4-PBS

Synonyme

ZFP161, Zfp 5, Zfp 161, ZF5, ZBTB14

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  WB analysis using 83909-4-RR (same clone as 83909-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83909-4-RR (ZFP161 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83909-4-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83909-4-RR (same clone as 83909-4-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ZFP161 / ZBTB14 Recombinant antibody (83909-4-RR, Clone:240853B12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83909-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83909-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83909-4-RR against Human ZFP161 / ZBTB14 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83909-4-PBS bindet in WB, FC (Intra), ELISA ZFP161 / ZBTB14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ZFP161 / ZBTB14 fusion protein Ag16125
• Vollständiger Name: zinc finger protein 161 homolog (mouse)
• Berechnetes Molekulargewicht: 449 aa, 51 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC110519
• Gene symbol: ZFP161
• Gene ID (NCBI): 7541
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZBTB14, also named as :ZFP161, ZNF478, is a 449 amino acid protein, which interacts with ZBTB21. ZBTB14, is a transcriptional activator of the dopamine transporter (DAT), binding it's promoter at the consensus sequence 5'-CCTGCACAGTTCACGGA-3'. ZBTB14 Binds to 5'-d(GCC)(n)-3' trinucleotide repeats in promoter regions and acts as a repressor of the FMR1 gene.