ZFP161 / ZBTB14 Rekombinanter Antikörper

ZFP161 / ZBTB14 Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240853G8

Kat-Nr. : 83909-5-RR

Synonyme

ZFP161, Zfp 5, Zfp 161, ZF5, ZBTB14

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  IF Staining of A549 using 83909-5-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ZFP161 / ZBTB14 antibody (83909-5-RR, Clone: 240853G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of MCF-7 using 83909-5-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ZFP161 / ZBTB14 Recombinant antibody (83909-5-RR, Clone:240853G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83909-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83909-5-RR against Human ZFP161 / ZBTB14 were performed. The affinity constant is 32.9 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: A549-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83909-5-RR bindet in IF/ICC, FC (Intra), ELISA ZFP161 / ZBTB14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ZFP161 / ZBTB14 fusion protein Ag16125
• Vollständiger Name: zinc finger protein 161 homolog (mouse)
• Berechnetes Molekulargewicht: 449 aa, 51 kDa
• GenBank-Zugangsnummer: BC110519
• Gene symbol: ZFP161
• Gene ID (NCBI): 7541
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZBTB14, also named as :ZFP161, ZNF478, is a 449 amino acid protein, which interacts with ZBTB21. ZBTB14, is a transcriptional activator of the dopamine transporter (DAT), binding it's promoter at the consensus sequence 5'-CCTGCACAGTTCACGGA-3'. ZBTB14 Binds to 5'-d(GCC)(n)-3' trinucleotide repeats in promoter regions and acts as a repressor of the FMR1 gene.