ZFP36 Polyklonaler Antikörper

ZFP36 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12737-1-PBS

Synonyme

TTP, Zfp-36, Zfp 36, Tristetraprolin, mRNA decay activator protein ZFP36

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  WB analysis using 12737-1-AP (same clone as 12737-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12737-1-AP (ZFP36 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12737-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 12737-1-AP (same clone as 12737-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12737-1-AP (ZFP36 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12737-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 12737-1-AP (same clone as 12737-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 12737-1-AP (ZFP36 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12737-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12737-1-AP (same clone as 12737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12737-1-AP (ZFP36 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12737-1-PBS in a different storage buffer formulation.

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  IHC staining of human bladder using 12737-1-AP (same clone as 12737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 12737-1-AP (ZFP36 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12737-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12737-1-AP (same clone as 12737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12737-1-AP (ZFP36 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12737-1-PBS in a different storage buffer formulation.

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  IHC staining of human bladder using 12737-1-AP (same clone as 12737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 12737-1-AP (ZFP36 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12737-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12737-1-AP (same clone as 12737-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZFP36 antibody (12737-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 12737-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12737-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ZFP36 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZFP36 fusion protein Ag3461
• Vollständiger Name: zinc finger protein 36, C3H type, homolog (mouse)
• Berechnetes Molekulargewicht: 326 aa, 34 kDa
• Beobachtetes Molekulargewicht: 37-44 kDa
• GenBank-Zugangsnummer: BC009693
• Gene symbol: ZFP36
• Gene ID (NCBI): 7538
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The expression of many cytokines is regulated post-transcriptionally by factors that modulate mRNA transport, translation, and stability. Much of this regulation occurs by the binding and stabilizing, or destabilizing, of cytokine mRNAs by proteins that recognize adenosine and uridine-rich elements (AREs) in untranslated regions of target transcripts. Zfp36 is a mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. It was demonstrated to physically interact with the p65 subunit of nuclear factor-κB leading to decreased nuclear import and diminished transcriptional activation mediated by nuclear factor-κB. It acted by specifically binding ARE-containing mRNAs and promoting their degradation, and has a crucial role in the post-transcriptional regulation of tumor necrosis factor (TNF). The calcualted molecular weight of ZFP36 is 34 kDa, but modified ZFP36 is about 40-45 kDa.