ZFP36 Monoklonaler Antikörper

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• KD/KO Validated

ZFP36 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A5E6

Kat-Nr. : 66938-1-PBS

Synonyme

TTP, Zfp 36, Tristetraprolin, mRNA decay activator protein ZFP36, Growth factor-inducible nuclear protein NUP475

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  WB analysis of HepG2 using 66938-1-Ig (same clone as 66938-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66938-1-Ig (same clone as 66938-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

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  WB analysis of NCI-H1299 using 66938-1-Ig (same clone as 66938-1-PBS)

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66938-1-Ig (same clone as 66938-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

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  WB analysis of 3T3-L1 using 66938-1-Ig (same clone as 66938-1-PBS)

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig lung using 66938-1-Ig (same clone as 66938-1-PBS)

  pig lung tissue were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 66938-1-Ig (same clone as 66938-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66938-1-Ig (ZFP36 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 66938-1-Ig (same clone as 66938-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66938-1-Ig (ZFP36 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66938-1-PBS bindet in WB, IHC, Indirect ELISA ZFP36 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ZFP36 fusion protein Ag28473
• Vollständiger Name: zinc finger protein 36, C3H type, homolog (mouse)
• Berechnetes Molekulargewicht: 326 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC009693
• Gene symbol: ZFP36
• Gene ID (NCBI): 7538
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The expression of many cytokines is regulated post-transcriptionally by factors that modulate mRNA transport, translation, and stability. Much of this regulation occurs by the binding and stabilizing, or destabilizing, of cytokine mRNAs by proteins that recognize adenosine and uridine-rich elements (AREs) in untranslated regions of target transcripts. Zfp36 is a mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. It was demonstrated to physically interact with the p65 subunit of nuclear factor-κB leading to decreased nuclear import and diminished transcriptional activation mediated by nuclear factor-κB. It acted by specifically binding ARE-containing mRNAs and promoting their degradation, and has a crucial role in the post-transcriptional regulation of tumor necrosis factor (TNF).