ZFP36L1 Monoklonaler Antikörper

ZFP36L1 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1H6G6

Kat-Nr. : 68038-1-Ig

Synonyme

Berg36, BRF1, Butyrate response factor 1, cMG1, EGF response factor 1, ERF 1, ERF1, Protein TIS11B, RNF162B, TIS11B, ZFP36L1

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  WB analysis using 68038-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68038-1-Ig (ZFP36L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of HSC-T6 using 68038-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68038-1-Ig (ZFP36L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 68038-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 68038-1-Ig (ZFP36L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 68038-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZFP36L1 antibody (68038-1-Ig, Clone: 1H6G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, A549-Zellen, HeLa-Zellen, PC-12-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68038-1-Ig bindet in WB, IF/ICC, ELISA ZFP36L1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ZFP36L1 fusion protein Ag30469
• Vollständiger Name: zinc finger protein 36, C3H type-like 1
• Berechnetes Molekulargewicht: 338 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC018340
• Gene symbol: ZFP36L1
• Gene ID (NCBI): 677
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

As a member of the Tristetraprolin (TTP) RNA binding protein family, ZFP36L1 regulate gene expression post-transcriptionally by promoting mRNA decay. In mammals, ZFP36L1 and its family members have been shown to regulate development, cell diferentiation, cell cycle, apoptosis and anti-cancer capability by targeting an extensive overlapping repertoire of mRNAs.