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ZFP36L2 Rekombinanter Antikörper

ZFP36L2 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242776C5

Kat-Nr. : 85154-4-PBS

Synonyme

BRF2, Butyrate response factor 2, EGF response factor 2, EGF-response factor 2, ERF 2

Filter:
  • Western Blot (WB) analysis of various lysates using ZFP36L2 Recombinant antibody (85154-4-RR)

    WB analysis using 85154-4-RR (same clone as 85154-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85154-4-RR (ZFP36L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85154-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ZFP36L2 Recombinant antibody (85154-4-RR)

    WB analysis using 85154-4-RR (same clone as 85154-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85154-4-RR (ZFP36L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85154-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZFP36L2 Recombinant antibody (85154-4-RR)

    IF Staining of HepG2 using 85154-4-RR (same clone as 85154-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZFP36L2 antibody (85154-4-RR, Clone: 242776C5 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85154-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85154-4-RR

    Affinity and Kinetic Characterization of 85154-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85154-4-RR against Human ZFP36L2 were performed. The affinity constant is 2.38 nM.

Geprüfte Anwendungen

Produktinformation

85154-4-PBS bindet in WB, IF/ICC, Indirect ELISA ZFP36L2 und zeigt Reaktivität mit Affe, human, Maus

Getestete Reaktivität Affe, human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen ZFP36L2 fusion protein Ag13515
Vollständiger Name zinc finger protein 36, C3H type-like 2
Berechnetes Molekulargewicht 51 kDa
Beobachtetes Molekulargewicht60 kDa
GenBank-ZugangsnummerBC005010
Gene symbol ZFP36L2
Gene ID (NCBI) 678
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZFP36L2 (Zinc finger protein 36 like 2) is an RNA-binding protein belonging to the ZFP36 family, which regulates gene expression at the post-transcriptional level by binding and destabilizing certain AU-rich elements (ARE) located in the 3′ untranslated regions (3′UTRs) of mRNA (PMID: 29518627). Moreover, ZFP36L2 is a key protein that controls S-phase progression in the case of genome instability (PMID: 29449217).