ZFP36L2 Rekombinanter Antikörper

ZFP36L2 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242776C5

Kat-Nr. : 85154-4-RR

Synonyme

BRF2, Butyrate response factor 2, EGF response factor 2, EGF-response factor 2, ERF 2

---

• 

  WB analysis using 85154-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85154-4-RR (ZFP36L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85154-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85154-4-RR (ZFP36L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 85154-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZFP36L2 antibody (85154-4-RR, Clone: 242776C5 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 85154-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85154-4-RR against Human ZFP36L2 were performed. The affinity constant is 2.38 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COS-7-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85154-4-RR bindet in WB, IF/ICC, ELISA ZFP36L2 und zeigt Reaktivität mit Affe, human, Maus

• Getestete Reaktivität: Affe, human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ZFP36L2 fusion protein Ag13515
• Vollständiger Name: zinc finger protein 36, C3H type-like 2
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC005010
• Gene symbol: ZFP36L2
• Gene ID (NCBI): 678
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZFP36L2 (Zinc finger protein 36 like 2) is an RNA-binding protein belonging to the ZFP36 family, which regulates gene expression at the post-transcriptional level by binding and destabilizing certain AU-rich elements (ARE) located in the 3′ untranslated regions (3′UTRs) of mRNA (PMID: 29518627). Moreover, ZFP36L2 is a key protein that controls S-phase progression in the case of genome instability (PMID: 29449217).