ZFP64 Polyklonaler Antikörper

ZFP64 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 17187-1-AP

Synonyme

Zfp 64, ZFP64, Zinc finger protein 338, ZNF338

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  WB analysis of mouse spleen using 17187-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17187-1-AP (ZFP64 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17187-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17187-1-AP (ZFP64 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below
• ChIP: See 3 publications below

Produktinformation

17187-1-AP bindet in WB, IF, ChIP, ELISA ZFP64 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZFP64 fusion protein Ag10971
• Vollständiger Name: zinc finger protein 64 homolog (mouse)
• Berechnetes Molekulargewicht: 681 aa, 75 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC041622
• Gene symbol: ZFP64
• Gene ID (NCBI): 55734
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZFP64 (also known as ZNF338) is a member of the Krüppel C2H2-type zinc finger family. ZFP64 is a coactivator of Notch1, mediating mesenchymal cell differentiation. ZFP64 expression was up-regulated in mouse peritoneal macrophages in response to stimulation with the TLR4 ligand LPS with a 2-fold increase. The MLL gene encoding the MLL fusion protein is easily activated by ZFP64. ZFP64 is essential for the continuous production of MLL fusion proteins in blood cancer and produces more cancer cells. Since ZFP64 deletion completely and specifically inhibits MLL fusion protein production and leukemia cascade growth, it is an ideal drug target.

Publikationen

Species	Application	Title
human	chIP	Cancer Cell; A Transcription Factor Addiction in Leukemia Imposed by the MLL Promoter Sequence.; Authors - Bin Lu
mouse	IF	Nat Neurosci; Neuronal activity rapidly reprograms dendritic translation via eIF4G2:uORF binding; Authors - Ezgi Hacisuleyman
human	WB,ChIP	Biol Direct; ZFP64 drives glycolysis-mediated stem cell-like properties and tumorigenesis in breast cancer; Authors - Jiayi Sun
human	ChIP	J Transl Med; Cancer-associated fibroblasts promote doxorubicin resistance in triple-negative breast cancer through enhancing ZFP64 histone lactylation to regulate ferroptosis; Authors - KeJing Zhang