ZFYVE16 Polyklonaler Antikörper

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• KD/KO Validated

ZFYVE16 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13118-2-PBS

Synonyme

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  WB analysis using 13118-2-AP (same clone as 13118-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 13118-2-AP (same clone as 13118-2-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 13118-2-AP (same clone as 13118-2-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 13118-2-AP (same clone as 13118-2-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 13118-2-AP (same clone as 13118-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 13118-2-AP (same clone as 13118-2-PBS)

  IP result of anti-ZFYVE16 (IP:13118-2-AP, 5ug; Detection:13118-2-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 13118-2-AP (same clone as 13118-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 13118-2-AP (same clone as 13118-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 13118-2-AP (same clone as 13118-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 13118-2-AP (same clone as 13118-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 13118-2-AP (same clone as 13118-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 13118-2-AP (same clone as 13118-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 13118-2-AP (same clone as 13118-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 13118-2-AP (same clone as 13118-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 13118-2-AP (same clone as 13118-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZFYVE16 antibody (13118-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). CL594-Phalloidin (red). DAPI (blue) This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13118-2-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA ZFYVE16 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZFYVE16 fusion protein Ag3811
• Vollständiger Name: zinc finger, FYVE domain containing 16
• Berechnetes Molekulargewicht: 1539 aa, 169 kDa
• Beobachtetes Molekulargewicht: 230-250 kDa
• GenBank-Zugangsnummer: BC032227
• Gene symbol: ZFYVE16
• Gene ID (NCBI): 9765
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Endofin (also known as ZFYVE16) is an endosomal FYVE domain protein implicated in regulating membrane trafficking. Endofin has been shown to localize to early endosomes. It co-localizes with SARA but does not associate with it or Smad2 and does not behave like SARA in affecting TGF-signaling. Northern blot analysis showed ZFYVE16 was widely expressed with high levels in kidney, placenta and lung. Overexpression of Endofin causes endosome aggregation. The gene of ZFYVE16 maps to chromosome 5q14, and encodes a 1539-amino acid protein with a molecular mass of 169 kDa. In addition, endogenous Endofin can also be detected as a band of 230-250 kDa. (PMID: 11546807)