ZG16 Polyklonaler Antikörper

ZG16 Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, IF-Fro, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 17397-1-AP

Synonyme

FLJ43571, FLJ92276, hZG16, JCLN, JCLN1

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  WB analysis of human colon using 17397-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 17397-1-AP (ZG16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 17397-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 17397-1-AP (ZG16 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17397-1-AP

  mouse colon tissue lysates were subjected to SDS PAGE followed by western blot with 17397-1-AP (ZG16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse pancreas using 17397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 17397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 17397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 17397-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 17397-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17397-1-AP (ZG16 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 17397-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17397-1-AP (ZG16 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse colon using 17397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 17397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse small intestine using 17397-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (17397-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse small intestine using 17397-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using ZG16 antibody (17397-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Kolongewebe, Maus-Kolongewebe
• Erfolgreiche Detektion in IHC: Maus-Pankreasgewebe, humanes Kolonkarzinomgewebe, humanes Pankreasgewebe, Maus-Kolongewebe, Maus-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IF-Fro: Maus-Dünndarmgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:600
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

17397-1-AP bindet in WB, IHC, IF-P, IF-Fro, ELISA ZG16 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZG16 fusion protein Ag11332
• Vollständiger Name: zymogen granule protein 16 homolog (rat)
• Berechnetes Molekulargewicht: 167 aa, 18 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC029149
• Gene symbol: ZG16
• Gene ID (NCBI): 653808
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zymogen granule protein 16 (ZG16) has a Jacalin-like lectin domain, which is mainly expressed by mucus-secreting cells, including goblet cells in the intestine. It's reported that ZG16 expression was significantly decreased in colorectal cancer compared to normal tissue. ZG16 gene expression and copy number variations (CNV) were associated with multiple molecular and clinicopathological features of CRC including MSI, MLH1 silencing and so on. It's found that ZG16 is negatively correlated with lymphatic invasive and distant metastasis. Besides, overexpression of ZG16 blocks PD-L1 expression in colorectal cancer, and promotes NK cells survival and proliferation. Very importantly, ZG16 suppresses colorectal tumor growth via the immune system. (PMID: 33360840, 21893569)

Publikationen

Species	Application	Title
human	WB	Nature; Co-transplantation of autologous Treg cells in a cell therapy for Parkinson's disease; Authors - Tae-Yoon Park
mouse,human	WB,IF	Adv Sci (Weinh); Gut Dysbiosis Drives Inflammatory Bowel Disease Through the CCL4L2-VSIR Axis in Glycogen Storage Disease; Authors - Jiaoli Lan