ZG16 Monoklonaler Antikörper

ZG16 Monoklonal Antikörper für WB, IHC, IF-P, IF-Fro, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A7B9

Kat-Nr. : 67389-1-PBS

Synonyme

1A7B9, FLJ43571, FLJ92276, hZG16, JCLN

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  WB analysis of mouse colon using 67389-1-Ig (same clone as 67389-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 67389-1-Ig (ZG16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

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  WB analysis of human colon using 67389-1-Ig (same clone as 67389-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 67389-1-Ig (ZG16 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

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  WB analysis of pig colon using 67389-1-Ig (same clone as 67389-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 67389-1-Ig (ZG16 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67389-1-Ig (ZG16 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67389-1-Ig (ZG16 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse small intestine using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3), ASCT2 antibody (20350-1-AP, green), CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-80008, Clone: 5H7, Magenta). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse small intestine using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3), ASCT2 antibody (20350-1-AP, green), CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-80008, Clone: 5H7, Magenta). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse small intestine using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3), ASCT2 antibody (20350-1-AP, green), CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-80008, Clone: 5H7, Magenta). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse small intestine using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse small intestine using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse small intestine using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CD163 antibody (83285-4-RR, Clone: 240233A3, red). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67389-1-PBS bindet in WB, IHC, IF-P, IF-Fro, Indirect ELISA ZG16 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ZG16 fusion protein Ag11332
• Vollständiger Name: zymogen granule protein 16 homolog (rat)
• Berechnetes Molekulargewicht: 167 aa, 18 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC029149
• Gene symbol: ZG16
• Gene ID (NCBI): 653808
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zymogen granule protein 16 (ZG16) has a Jacalin-like lectin domain, which is mainly expressed by mucus-secreting cells, including goblet cells in the intestine. It's reported that ZG16 expression was significantly decreased in colorectal cancer compared to normal tissue. ZG16 gene expression and copy number variations (CNV) were associated with multiple molecular and clinicopathological features of CRC including MSI, MLH1 silencing and so on. It's found that ZG16 is negatively correlated with lymphatic invasive and distant metastasis. Besides, overexpression of ZG16 blocks PD-L1 expression in colorectal cancer, and promotes NK cells survival and proliferation. Very importantly, ZG16 suppresses colorectal tumor growth via the immune system. (PMID: 33360840, 21893569)