ZG16 Monoklonaler Antikörper

ZG16 Monoklonal Antikörper für IF-P

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF-P

Konjugation

CoraLite® Plus 594 Fluorescent Dye

CloneNo.

1A7B9

Kat-Nr. : CL594-67389

Synonyme

FLJ43571, FLJ92276, hZG16, JCLN, JCLN1, Secretory lectin ZG16, ZG16, ZG16A, Zymogen granule protein 16

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  IF Staining of mouse small intestine using CL594-67389

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite® Plus 594 ZG16 antibody (CL594-67389, Clone: 1A7B9 ) at dilution of 1:100, CoraLite® Plus 488 PIGR antibody (CL488-22024, green), Villin antibody (16488-1-AP, Magenta).

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  IF Staining of mouse small intestine using CL594-67389

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite® Plus 594 ZG16 antibody (CL594-67389, Clone: 1A7B9 ) at dilution of 1:100, CoraLite® Plus 488 PIGR antibody (CL488-22024, green), Villin antibody (16488-1-AP, Magenta).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maus-Dünndarmgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67389 bindet in IF-P ZG16 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ZG16 fusion protein Ag11332
• Vollständiger Name: zymogen granule protein 16 homolog (rat)
• Berechnetes Molekulargewicht: 167 aa, 18 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC029149
• Gene symbol: ZG16
• Gene ID (NCBI): 653808
• Konjugation: CoraLite® Plus 594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 594 nm / 615 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zymogen granule protein 16 (ZG16) has a Jacalin-like lectin domain, which is mainly expressed by mucus-secreting cells, including goblet cells in the intestine. It's reported that ZG16 expression was significantly decreased in colorectal cancer compared to normal tissue. ZG16 gene expression and copy number variations (CNV) were associated with multiple molecular and clinicopathological features of CRC including MSI, MLH1 silencing and so on. It's found that ZG16 is negatively correlated with lymphatic invasive and distant metastasis. Besides, overexpression of ZG16 blocks PD-L1 expression in colorectal cancer, and promotes NK cells survival and proliferation. Very importantly, ZG16 suppresses colorectal tumor growth via the immune system. (PMID: 33360840, 21893569)