ZHX1 Polyklonaler Antikörper

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ZHX1 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13903-1-PBS

Synonyme

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• 

  WB analysis of HeLa using 13903-1-AP (same clone as 13903-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 13903-1-AP (same clone as 13903-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 13903-1-AP (same clone as 13903-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 13903-1-AP (same clone as 13903-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 13903-1-AP (same clone as 13903-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 13903-1-AP (same clone as 13903-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 13903-1-AP (same clone as 13903-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 13903-1-AP (same clone as 13903-1-PBS)

  IP result of anti-ZHX1 (IP:13903-1-AP, 4ug; Detection:13903-1-AP 1:500) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13903-1-PBS bindet in WB, IP, Indirect ELISA ZHX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZHX1 fusion protein Ag4874
• Vollständiger Name: zinc fingers and homeoboxes 1
• Berechnetes Molekulargewicht: 873 aa, 98 kDa
• Beobachtetes Molekulargewicht: 100-120 kDa
• GenBank-Zugangsnummer: BC040481
• Gene symbol: ZHX1
• Gene ID (NCBI): 11244
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Binding of the nuclear factor-Y complex (NF-Y) to the inverted CCAAT-box interferes with transcription activation through nucleosome reorganization. The three homologous proteins forming the zinc-fingers and homeoboxes (ZHX) family interact with the activation domain of NF-Ya to repress transcription [PMID:19348505,20509910]. Human zinc-fingers and homeoboxes 1 (ZHX1) was cloned as a protein that interacts with the activation domain (AD) of the A subunit of nuclear factor-Y (NF-YA) [PMID:12237128].