ZIC3 Polyklonaler Antikörper

ZIC3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 21717-1-AP

Synonyme

ZNF203, Zinc finger protein ZIC 3

---

• 

  WB analysis of Neuro-2a using 21717-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 21717-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 21717-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 21717-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 21717-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 21717-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 21717-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat brain using 21717-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 21717-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 21717-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 21717-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 21717-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21717-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, Maus-Cerebellum-Gewebe, SH-SY5Y-Zellen, Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Urothelkarzinomgewebe, humanes Schilddrüsenkarzinomgewebe, Maushirngewebe, Maus-Cerebellum-Gewebe, Rattenhirngewebe, Ratten-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21717-1-AP bindet in WB, IHC, ELISA ZIC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZIC3 fusion protein Ag16354
• Vollständiger Name: Zic family member 3 (odd-paired homolog, Drosophila)
• Berechnetes Molekulargewicht: 467 aa, 51 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC113395
• Gene symbol: ZIC3
• Gene ID (NCBI): 7547
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other