ZIM3 Polyklonaler Antikörper

ZIM3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25200-1-AP

Synonyme

ZIM 3, Zinc finger protein 657, ZNF657

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  WB analysis of COLO 320 using 25200-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25200-1-AP (ZIM3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 25200-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25200-1-AP (ZIM3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25200-1-AP bindet in WB, ELISA ZIM3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZIM3 fusion protein Ag18411
• Vollständiger Name: zinc finger, imprinted 3
• Berechnetes Molekulargewicht: 472 aa, 57 kDa
• Beobachtetes Molekulargewicht: 55-65 kDa
• GenBank-Zugangsnummer: BC114503
• Gene symbol: ZIM3
• Gene ID (NCBI): 114026
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZIM3 (Zinc Finger Imprinted 3), also known as ZNF657 or ZNF264, is a protein containing zinc finger and KRAB domains. It belongs to the Krüppel C2H2-type zinc finger protein family and comprises 11 C2H2-type zinc fingers and 1 KRAB domain. ZIM3 influences chromatin organization by binding to the KAP1 protein, thereby regulating gene transcription. ZIM3 is predicted to have DNA-binding transcription factor activity and is involved in the transcriptional regulation of RNA polymerase II. Additionally, ZIM3 plays a core regulatory role in human major zygotic genome activation (ZGA).