ZIP8 Polyklonaler Antikörper

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ZIP8 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20459-1-PBS

Synonyme

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  WB analysis of mouse lung using 20459-1-AP (same clone as 20459-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20459-1-AP (ZIP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-937 using 20459-1-AP (same clone as 20459-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 20459-1-AP (ZIP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 20459-1-AP (same clone as 20459-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20459-1-AP (ZIP8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 20459-1-AP (same clone as 20459-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20459-1-AP (ZIP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse liver using 20459-1-AP (same clone as 20459-1-PBS)

  IP result of anti-ZIP8 (IP:20459-1-AP, 4ug; Detection:20459-1-AP 1:500) with mouse liver tissue lysate 6000ug. This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:800 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20459-1-AP (ZIP8 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20459-1-AP (ZIP8 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20459-1-AP (ZIP8 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20459-1-AP (ZIP8 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 20459-1-AP (ZIP8 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20459-1-PBS bindet in WB, IHC, IP, Indirect ELISA ZIP8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZIP8 fusion protein Ag14292
• Vollständiger Name: solute carrier family 39 (zinc transporter), member 8
• Berechnetes Molekulargewicht: 460 aa, 50 kDa
• Beobachtetes Molekulargewicht: 42-46 kDa, 75-90 kDa
• GenBank-Zugangsnummer: BC012125
• Gene symbol: ZIP8
• Gene ID (NCBI): 64116
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC39A8, also known as ZIP8, belongs to the ZIP family of metal ion transporters which function to transport zinc and/or other metal ion substrates from the extracellular space or organellar lumen into the cytoplasm. Recently it was found that ZIP8 expression is upregulated in human monocytes in response to LPS, TNF-α, and live bacteria, facilitating cytoprotection during the early inflammation. Besides zinc ZIP8 can also transport cadmium and manganese efficiently. It is predicted that ZIP8 contains 3 potential N-linked glycosylation sites and is subject to glycosylation, which may account for the presences of multiple molecular weights, such as 43 kDa, 49 kDa, 60 kDa, 75-90 kDa, 150 kDa, and 200 kDa.