ZNF143 Polyklonaler Antikörper

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• KD/KO Validated

ZNF143 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 16618-1-AP

Synonyme

hStaf, pHZ 1, SBF, SPH binding factor, STAF, zinc finger protein 143, ZNF143

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 16618-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16618-1-AP (ZNF143 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16618-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16618-1-AP (ZNF143 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16618-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16618-1-AP (ZNF143 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 7 publications below

Produktinformation

16618-1-AP bindet in WB, IHC, IF, CoIP, ChIP, ELISA ZNF143 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZNF143 fusion protein Ag9914
• Vollständiger Name: zinc finger protein 143
• Berechnetes Molekulargewicht: 626 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC020219
• Gene symbol: ZNF143
• Gene ID (NCBI): 7702
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zinc finger protein143(ZNF143) contains a separete acidic activation and 7 zinc finger domains. It's a transcription factor that required for activation of the majority of vertebrate snRNA and snRNA-type genes, which transcribed by RNA pol II and pol III. Also, it binds to the SPH motif of small nuclear RNA (snRNA) gene promoters and participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8

Publikationen

Species	Application	Title
mouse	chIP	Mol Cell; Maintenance of CTCF- and Transcription Factor-Mediated Interactions from the Gametes to the Early Mouse Embryo.; Authors - Yoon Hee Jung
human	chIP	Mol Cell; Architectural Proteins and Pluripotency Factors Cooperate to Orchestrate the Transcriptional Response of hESCs to Temperature Stress.; Authors - Xiaowen Lyu
mouse	CoIP	Nat Commun; Mutations in Hcfc1 and Ronin result in an inborn error of cobalamin metabolism and ribosomopathy.; Authors - Tiffany Chern
human	ChIP	Proc Natl Acad Sci U S A; Cooperative transcription factor associations discovered using regulatory variation.; Authors - Karczewski Konrad J KJ
human	chIP	Cancer Res; ZNF143-Mediated H3K9 Trimethylation Upregulates CDC6 by Activating MDIG in Hepatocellular Carcinoma.; Authors - Lili Zhang ; KD Validated
human	WB, ChIP	Cell Rep; Host Cell Factor-1 Recruitment to E2F-Bound and Cell-Cycle-Control Genes Is Mediated by THAP11 and ZNF143.; Authors - J Brandon Parker ; KD Validated