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ZNF148 Rekombinanter Antikörper

ZNF148 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241632D8

Kat-Nr. : 84338-7-PBS

Synonyme

Transcription factor ZBP 89, pHZ 52, HT BETA, BFCOL1, BERF 1

Filter:
  • Western Blot (WB) analysis of various lysates using ZNF148 Recombinant antibody (84338-7-RR)

    WB analysis using 84338-7-RR (same clone as 84338-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84338-7-RR (ZNF148 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84338-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84338-7-RR

    Affinity and Kinetic Characterization of 84338-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84338-7-RR against Human ZNF148 were performed. The affinity constant is 1.85 nM.

Geprüfte Anwendungen

Produktinformation

84338-7-PBS bindet in WB, Indirect ELISA ZNF148 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen ZNF148 fusion protein Ag35742
Vollständiger Name zinc finger protein 148
Beobachtetes Molekulargewicht103 kDa
GenBank-ZugangsnummerBC035591
Gene symbol ZNF148
Gene ID (NCBI) 7707
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Kruppel-like zinc finger protein 148 (ZNF148), also known as β enolase repressor factor 1 (BERF1, ZBP-89, and BFCOL1), encoded by this gene is a member of the Kruppel family of zinc finger DNA binding proteins. ZNF148 participates in the regulation of cell proliferation and death, and is expressed at lower levels in mammalian somatic cells, and highly expressed in tumor cells. Previous studies show that there was a significant correlation between ZNF148 expression and the invasion and metastasis of colorectal tumors. ZNF148 has two alternative splicing isoforms, which are ZNF148FL and ZNF148ΔN. ZNF148FL contains a complete 794 amino acids, ZNF148ΔN lacks the amino-terminal 129 amino acids, and the mechanisms involved in the generation of these two splicing isoforms are not yet clear (PMID: 30463804). Thus, ZNF148 can be observed to have two molecular weights near 103 kDa.