ZNF148 Rekombinanter Antikörper

ZNF148 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241632D8

Kat-Nr. : 84338-7-RR

Synonyme

Transcription factor ZBP 89, pHZ 52, HT BETA, BFCOL1, BERF 1

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  WB analysis using 84338-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84338-7-RR (ZNF148 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84338-7-RR

  Biolayer interferometry (BLl) kinetic assays of 84338-7-RR against Human ZNF148 were performed. The affinity constant is 1.85 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HCT 116-Zellen, HeLa-Zellen, HepG2-Zellen, MDA-MB-231-Zellen, NIH/3T3-Zellen, T-47D-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84338-7-RR bindet in WB, ELISA ZNF148 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ZNF148 fusion protein Ag35742
• Vollständiger Name: zinc finger protein 148
• Beobachtetes Molekulargewicht: 103 kDa
• GenBank-Zugangsnummer: BC035591
• Gene symbol: ZNF148
• Gene ID (NCBI): 7707
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Kruppel-like zinc finger protein 148 (ZNF148), also known as β enolase repressor factor 1 (BERF1, ZBP-89, and BFCOL1), encoded by this gene is a member of the Kruppel family of zinc finger DNA binding proteins. ZNF148 participates in the regulation of cell proliferation and death, and is expressed at lower levels in mammalian somatic cells, and highly expressed in tumor cells. Previous studies show that there was a significant correlation between ZNF148 expression and the invasion and metastasis of colorectal tumors. ZNF148 has two alternative splicing isoforms, which are ZNF148FL and ZNF148ΔN. ZNF148FL contains a complete 794 amino acids, ZNF148ΔN lacks the amino-terminal 129 amino acids, and the mechanisms involved in the generation of these two splicing isoforms are not yet clear (PMID: 30463804). Thus, ZNF148 can be observed to have two molecular weights near 103 kDa.