ZNF154 Polyklonaler Antikörper

ZNF154 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22161-1-AP

Synonyme

KIAA2003, pHZ 92, zinc finger protein 154, ZNF154

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Galerie der Validierungsdaten

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  WB analysis of COS-7 using 22161-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 22161-1-AP (ZNF154 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22161-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22161-1-AP (ZNF154 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22161-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22161-1-AP (ZNF154 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 22161-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22161-1-AP (ZNF154 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COS-7-Zellen, A549-Zellen, HEK-293-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

22161-1-AP bindet in WB, ELISA ZNF154 und zeigt Reaktivität mit Affe, human

• Getestete Reaktivität: Affe, human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZNF154 fusion protein Ag17812
• Vollständiger Name: zinc finger protein 154
• Berechnetes Molekulargewicht: 437 aa, 50 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC152404
• Gene symbol: ZNF154
• Gene ID (NCBI): 7710
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sites that display recurrent, aberrant DNA methylation in cancer represent potential biomarkers for screening and diagnostics. Previously, The hypermethylation at the ZNF154 CpG island in 15 solid epithelial tumor types from 13 different organs. The magnitude and reproducibility of the ZNF154 hypermethylation signal across five solid tumor types reinforces the potential of this site as a biomarker for circulating tumor DNA (ctDNA)(PMID: 26857064 ). The calculated molecular weight of ZNF154 is 50 kDa, but the modified ZNF154 protein is about 55-60 kDa.