ZNF217 Rekombinanter Antikörper

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ZNF217 Rekombinant Antikörper für WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241217E3

Kat-Nr. : 84276-1-PBS

Synonyme

ZFP217, 241217E3, ZABC1, Zinc finger protein 217

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  WB analysis using 84276-1-RR (same clone as 84276-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84276-1-RR (ZNF217 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84276-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 84276-1-RR (same clone as 84276-1-PBS)

  WB result of ZNF217 antibody (84276-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZNF217 transfected HEK-293 cells. This data was developed using the same antibody clone with 84276-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01357-1

  Sandwich ELISA standard curve of MP01357-1, Human ZNF217 Recombinant Matched Antibody Pair - PBS only. 84276-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag19453. 84276-2-PBS was HRP conjugated as the detection antibody. Range: 6.25-400 ng/mL

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  Sample test of MP01357-1

  The mean ZNF217 concentration was determined to be 885 ng/mL in HepG2 cell extract based on a 1.3 mg/mL extract load.

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  IF Staining of HepG2 using 84276-1-RR (same clone as 84276-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNF217 antibody (84276-1-RR, Clone: 241217E3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84276-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84276-1-RR (same clone as 84276-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNF217 antibody (84276-1-RR, Clone: 241217E3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84276-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84276-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84276-1-RR against Human ZNF217 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84276-1-PBS bindet in WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test ZNF217 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ZNF217 fusion protein Ag19453
• Vollständiger Name: zinc finger protein 217
• Berechnetes Molekulargewicht: 1048 aa, 115 kDa
• Beobachtetes Molekulargewicht: ~ 130 kDa
• GenBank-Zugangsnummer: BC113427
• Gene symbol: ZNF217
• Gene ID (NCBI): 7764
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zinc finger protein 217 (ZNF217) is a member of the Krüppel-like factor transcription factor family. ZNF217 possesses a characteristic structure of zinc finger motifs and plays a crucial role in regulating the biological activities of cells. ZNF217 binds to DNA through its zinc fingers thereby targeting potential protein binding partners to specific sites in the genome. Recent findings have revealed that ZNF217 is strongly associated with multiple aspects of cancer progression, impacting patient prognosis. PMID: 38259608