ZNF217 Rekombinanter Antikörper

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• KD/KO Validated

ZNF217 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241217E3

Kat-Nr. : 84276-1-RR

Synonyme

ZFP217, 241217E3, ZABC1, Zinc finger protein 217

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  WB analysis using 84276-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84276-1-RR (ZNF217 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 84276-1-RR

  WB result of ZNF217 antibody (84276-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZNF217 transfected HEK-293 cells.

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  IF Staining of HepG2 using 84276-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNF217 antibody (84276-1-RR, Clone: 241217E3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 84276-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNF217 antibody (84276-1-RR, Clone: 241217E3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84276-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84276-1-RR against Human ZNF217 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Caco-2-Zellen, HEK-293-Zellen, LNCaP-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84276-1-RR bindet in WB, IF/ICC, ELISA ZNF217 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ZNF217 fusion protein Ag19453
• Vollständiger Name: zinc finger protein 217
• Berechnetes Molekulargewicht: 1048 aa, 115 kDa
• Beobachtetes Molekulargewicht: ~ 130 kDa
• GenBank-Zugangsnummer: BC113427
• Gene symbol: ZNF217
• Gene ID (NCBI): 7764
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zinc finger protein 217 (ZNF217) is a member of the Krüppel-like factor transcription factor family. ZNF217 possesses a characteristic structure of zinc finger motifs and plays a crucial role in regulating the biological activities of cells. ZNF217 binds to DNA through its zinc fingers thereby targeting potential protein binding partners to specific sites in the genome. Recent findings have revealed that ZNF217 is strongly associated with multiple aspects of cancer progression, impacting patient prognosis. PMID: 38259608