ZNF23 Polyklonaler Antikörper

ZNF23 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 21322-1-AP

Synonyme

Zinc finger protein KOX16, Zinc finger protein 612, Zinc finger protein 359, Zinc finger protein 23, Zfp612

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  WB analysis of HepG2 using 21322-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21322-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21322-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 21322-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 21322-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 21322-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 21322-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 21322-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21322-1-AP

  IP result of anti-ZNF23 (IP:21322-1-AP, 4ug; Detection:21322-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IF Staining of HepG2 using 21322-1-AP

  Immunofluorescent analysis of HepG2 cells, using ZNF23 antibody 21322-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A2780-Zellen, HeLa-Zellen, Maushirngewebe, Mauslungengewebe, Mausmilzgewebe, SKOV-3-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

21322-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ZNF23 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZNF23 fusion protein Ag14755
• Vollständiger Name: zinc finger protein 23 (KOX 16)
• Berechnetes Molekulargewicht: 643 aa, 73 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC007256
• Gene symbol: ZNF23
• Gene ID (NCBI): 7571
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IHC	Cell Physiol Biochem; ZNF23 Suppresses Cutaneous Melanoma Cell Malignancy via Mitochondria-Dependent Pathway.; Authors - Xin Zhang