ZNF238/ZBTB18 Rekombinanter Antikörper

ZNF238/ZBTB18 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

251389B9

Kat-Nr. : 86544-3-RR

Synonyme

ZNF238, 58 kDa repressor protein, C2H2 171, RP58, TAZ 1

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  WB analysis using 86544-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86544-3-RR (ZNF238 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86544-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86544-3-RR against Human ZNF238/ZBTB18 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Cerebellum-Gewebe, Ratten-Cerebellum-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86544-3-RR bindet in WB, ELISA ZNF238/ZBTB18 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ZNF238/ZBTB18 fusion protein Ag3406
• Vollständiger Name: zinc finger protein 238
• Berechnetes Molekulargewicht: 531 aa, 59 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC036677
• Gene symbol: ZNF238/ZBTB18
• Gene ID (NCBI): 10472
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZNF238 is a member of the BTB/POZ-ZF protein family, which involve in development and cancer formation, for example BCL-6, PLZF, and HIC-1. It's a transcriptional repressor involve in myogenesis and brain development. By directly repressing the expression of two skeletal myogenesis inhibitors, ID2 and ID3, ZNF238 plays a key role in myogenesis. It can control cell division of progenitor cells and regulating the survival of postmitotic cortical neurons. Besides, ZNF238 involves in the organization of nuclear chromosomes, for its specific binding to the consensus DNA sequence that contains the E box core, and recruiting chromatin remodeling multi-protein complex. ZNF238 proteins has apparent molecular masses of 60 and 48 kD. Specific binding is found for a 60-kDa band which corresponds to the full length of RP58 protein. In addition, a 48-kDa band, thought to be the truncated form 2 is detected (PMID: 9756912).