ZNF24 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ZNF24 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 11219-1-AP

Synonyme

Zinc finger protein 191, Zinc finger and SCAN domain-containing protein 3, Zfp191, RSG-A, RSG A

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  WB analysis of HeLa using 11219-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11219-1-AP (ZNF24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11219-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11219-1-AP (ZNF24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11219-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11219-1-AP (ZNF24 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 11219-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 11219-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 11219-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 11219-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 11219-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 11219-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 11219-1-AP

  Immunofluorescent analysis of HeLa cells using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

11219-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ZNF24 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZNF24 fusion protein Ag1724
• Vollständiger Name: zinc finger protein 24
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC016801
• Gene symbol: ZNF24
• Gene ID (NCBI): 7572
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zinc finger protein 24 (ZNF24; also known as KOX17, Zfp191 or ZNF191) is a member of the Kruppel-like zinc finger (ZF) transcription factor family, which plays a critical role in cell proliferation and differentiation. There're two isoforms with calculated MW 42KDa and 22KDa. ZNF24 protein can be modificated with SUMO.

Publikationen

Species	Application	Title
human	WB,IHC	Cell Death Dis; LncRNA ZNFTR functions as an inhibitor in pancreatic cancer by modulating ATF3/ZNF24/VEGFA pathway.; Authors - Wei Li ; KD Validated
human	IHC,WB,IF	Front Oncol; ZNF24 regulates the progression of KRAS mutant lung adenocarcinoma by promoting SLC7A5 translation; Authors - Daqi Jia
human	IHC	Exp Cell Res; Tumor-suppressive zinc finger protein 24 (ZNF24) sensitizes colorectal cancer cells to 5-fluorouracil by inhibiting the Wnt pathway and activating the p53 signaling; Authors - Fanqi Meng
human	WB,IP	Cell Death Dis; The deubiquitinase YOD1 suppresses tumor progression by stabilizing ZNF24 in clear cell renal carcinoma; Authors - Ji Liu
mouse	IHC	Oncogene; Crosstalk between SUMOylation and ubiquitination controls the stability of transcription factor zinc finger protein 24: a novel antitumor mechanism in bladder cancer; Authors - Xiaosong Wei