ZNF251 Polyklonaler Antikörper

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• KD/KO Validated

ZNF251 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 25601-1-AP

Synonyme

zinc finger protein 251, ZNF251

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  WB analysis of mouse testis using 25601-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25601-1-AP (ZNF251 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 25601-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25601-1-AP (ZNF251 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25601-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25601-1-AP (ZNF251 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 25601-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25601-1-AP (ZNF251 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of SKOV-3 using 25601-1-AP

  Immunofluorescent analysis of SKOV-3 cells using 25601-1-AP (ZNF251 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, HepG2-Zellen, Maus-Skelettmuskelgewebe, SKOV-3-Zellen
• Erfolgreiche Detektion in IF/ICC: SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below

Produktinformation

25601-1-AP bindet in WB, IF/ICC, ELISA ZNF251 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZNF251 fusion protein Ag22347
• Vollständiger Name: zinc finger protein 251
• Berechnetes Molekulargewicht: 671 aa, 76 kDa
• Beobachtetes Molekulargewicht: 70-76 kDa
• GenBank-Zugangsnummer: BC112137
• Gene symbol: ZNF251
• Gene ID (NCBI): 90987
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Res Sq; Haploinsufficiency of ZNF251 causes DNA-PKcs-dependent resistance to PARP inhibitors in BRCA1-mutated cancer cells; Authors - Huan Li ; KD Validated
human	WB	Cancer Lett; ZNF251 haploinsufficiency confers PARP inhibitors resistance in BRCA1-mutated cancer cells through activation of homologous recombination; Authors - Huan Li ; KD Validated