ZNF326 Polyklonaler Antikörper

ZNF326 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (2)

Kat-Nr. : 25147-1-AP

Synonyme

ZAN75, Zfp326, zinc finger protein 326, ZNF326

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• 

  WB analysis of Jurkat using 25147-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25147-1-AP (ZNF326 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25147-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25147-1-AP (ZNF326 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 25147-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25147-1-AP (ZNF326 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25147-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25147-1-AP (ZNF326 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 25147-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25147-1-AP (ZNF326 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse liver using 25147-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25147-1-AP (ZNF326 Antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 25147-1-AP

  Immunofluorescent analysis of HeLa cells using 25147-1-AP (ZNF326 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, MCF-7-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

25147-1-AP bindet in WB, IHC, IF/ICC, ELISA ZNF326 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZNF326 fusion protein Ag18433
• Vollständiger Name: zinc finger protein 326
• Berechnetes Molekulargewicht: 582 aa, 66 kDa
• Beobachtetes Molekulargewicht: 66-70 kDa
• GenBank-Zugangsnummer: BC090866
• Gene symbol: ZNF326
• Gene ID (NCBI): 284695
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	Sci Rep; TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.; Authors - S Prpar Mihevc
mouse	WB	Toxicol Sci; LncRNA NR_030777 Alleviates Paraquat-induced Neurotoxicity by Regulating Zfp326 and Cpne5.; Authors - Hongyu Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (12-16-2024)

Great! Znf326 is visible (below 75 kDa), as well as other weak non-specific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: RKO

ZNF326 Antibody Western Blot validation (1:2000 dilution) in RKO (Cat no:25147-1-AP)

FH

Tsimafei (Verified Customer) (08-07-2024)

Antibody detect the band of the correct size

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: U2OS

ZNF326 Antibody Western Blot validation (1:2000 dilution) in U2OS (Cat no:25147-1-AP)