ZNF394 Polyklonaler Antikörper

ZNF394 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 25785-1-AP

Synonyme

ZKSCAN14

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  WB analysis of mouse brain using 25785-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25785-1-AP (ZNF394 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 25785-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25785-1-AP (ZNF394 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25785-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25785-1-AP (ZNF394 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 25785-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25785-1-AP (ZNF394 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human urothelial carcinoma using 25785-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 25785-1-AP (ZNF394 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, fetales humanes Hirngewebe, HepG2-Zellen, Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Urothelkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25785-1-AP bindet in WB, IHC, ELISA ZNF394 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZNF394 fusion protein Ag22655
• Vollständiger Name: zinc finger protein 394
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC051847
• Gene symbol: ZNF394
• Gene ID (NCBI): 84124
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZNF394 (zinc finger protein 394) is a C2H2-type zinc finger protein belonging to the Krüppel C2H2-type zinc finger protein family. The protein contains three distinct domains: the N-terminus has two domains, LER and KRAB, and the C-terminus has seven C2H2 zinc finger domains. ZNF394 is predicted to have DNA-binding transcription factor activity and is involved in the transcriptional regulation of RNA polymerase II. Additionally, ZNF394, together with ZIM3, plays a key role in human embryonic development at the 8-cell stage by inhibiting the expression of genes specific to the 4-cell stage, thereby promoting embryonic development.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Simone (Verified Customer) (04-25-2022)

From 6 well plate pellet, lysed with 1ml NP40 buffer

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T cells