ZNF395 Polyklonaler Antikörper

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ZNF395 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11759-1-PBS

Synonyme

HD-regulating factor 2, HDBP-2, HDBP2, HDBP 2, HD regulating factor 2

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  WB analysis of human brain using 11759-1-AP (same clone as 11759-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11759-1-AP (ZNF395 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11759-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 11759-1-AP (same clone as 11759-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11759-1-AP (ZNF395 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11759-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11759-1-AP (same clone as 11759-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11759-1-AP (ZNF395 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11759-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 11759-1-AP (same clone as 11759-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11759-1-AP (ZNF395 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11759-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11759-1-AP (same clone as 11759-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZNF395 antibody (11759-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11759-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11759-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ZNF395 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZNF395 fusion protein Ag2355
• Vollständiger Name: zinc finger protein 395
• Berechnetes Molekulargewicht: 513 aa, 55 kDa
• Beobachtetes Molekulargewicht: 62 kDa, 72 kDa
• GenBank-Zugangsnummer: BC001237
• Gene symbol: ZNF395
• Gene ID (NCBI): 55893
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZNF395 is a transcription factors binding to a 7bp GC rich sequence which resides in triplicate at intervals of 13bp within and proximal to the -20bp direct repeat sequences of the Htt promoter. It can bind to regulatory regions in papillomaviruses (PV) and subsequently called the protein papillomavirus binding factor (PBF) [PMID: 11853404]. It contains conserved regions CR1, CR2 and CR3, a domain rich in serines and prolines and have the potential to form a zinc-finger structure, and the C-terminal CR3 is responsible for DNA-binding [PMID: 17897615].