ZNF451 Polyklonaler Antikörper

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• KD/KO Validated

ZNF451 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25228-1-PBS

Synonyme

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  WB analysis of HeLa using 25228-1-AP (same clone as 25228-1-PBS)

  WB result of ZNF451 antibody (25228-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZNF451 transfected HeLa cells. This data was developed using the same antibody clone with 25228-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 25228-1-AP (same clone as 25228-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 25228-1-AP (ZNF451 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25228-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 25228-1-AP (same clone as 25228-1-PBS)

  IP result of anti-ZNF451 (IP:25228-1-AP, 4ug; Detection:25228-1-AP 1:300) with HeLa cells lysate 1080 ug. This data was developed using the same antibody clone with 25228-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 25228-1-AP (same clone as 25228-1-PBS)

  IP result of anti-ZNF451 (IP:25228-1-AP, 4ug; Detection:25228-1-AP 1:300) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 25228-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 25228-1-AP (same clone as 25228-1-PBS)

  IP result of anti-ZNF451 (IP:25228-1-AP, 4ug; Detection:25228-1-AP 1:300) with HeLa cells lysate 1080 ug. This data was developed using the same antibody clone with 25228-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 25228-1-AP (same clone as 25228-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25228-1-AP (ZNF451 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25228-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 25228-1-AP (same clone as 25228-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25228-1-AP (ZNF451 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25228-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 25228-1-AP (same clone as 25228-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 25228-1-AP (ZNF451 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25228-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25228-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA ZNF451 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZNF451 fusion protein Ag18346
• Vollständiger Name: zinc finger protein 451
• Berechnetes Molekulargewicht: 1061 aa, 121 kDa
• Beobachtetes Molekulargewicht: 121 kDa
• GenBank-Zugangsnummer: BC021712
• Gene symbol: ZNF451
• Gene ID (NCBI): 26036
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.