ZNF618 Polyklonaler Antikörper

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• KD/KO Validated

ZNF618 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31972-1-PBS

Synonyme

KIAA1952, Zinc finger protein 618

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  WB analysis using 31972-1-AP (same clone as 31972-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31972-1-AP (ZNF618 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31972-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 31972-1-AP (same clone as 31972-1-PBS)

  WB result of ZNF618 antibody (31972-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZNF618 transfected SH-SY5Y cells. This data was developed using the same antibody clone with 31972-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 31972-1-AP (same clone as 31972-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31972-1-AP (ZNF618 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31972-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 31972-1-AP (same clone as 31972-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 31972-1-AP (ZNF618 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31972-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 31972-1-AP (same clone as 31972-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ZNF618 antibody (31972-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 31972-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

31972-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ZNF618 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZNF618 fusion protein Ag36487
• Vollständiger Name: zinc finger protein 618
• Berechnetes Molekulargewicht: 105kDa，954aa
• Beobachtetes Molekulargewicht: 100-113 kDa
• GenBank-Zugangsnummer: NM_001318042.1
• Gene symbol: ZNF618
• Gene ID (NCBI): 114991
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zinc finger protein 618 (ZNF618) is a key protein associated with DNA methylation and acts as a specific 5hmC reader in vivo to regulate UHRF2 function by promoting UHRF2 chromatin localization. In addition, ZNF618 is not only associated with the induction, maintenance or progression of female breast cancer, but is also up-regulated in lung cancer cells.