ZNF618 Rekombinanter Antikörper

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• KD/KO Validated

ZNF618 Rekombinant Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242566D9

Kat-Nr. : 84998-1-PBS

Synonyme

242566D9, KIAA1952, Zinc finger protein 618

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  WB analysis using 84998-1-RR (same clone as 84998-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84998-1-RR (ZNF618 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84998-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 84998-1-RR (same clone as 84998-1-PBS)

  WB result of ZNF618 antibody (84998-1-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZNF618 transfected SH-SY5Y cells. This data was developed using the same antibody clone with 84998-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84998-1-RR (same clone as 84998-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84998-1-RR (ZNF618 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84998-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 84998-1-RR (same clone as 84998-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ZNF618 antibody (84998-1-RR, Clone: 242566D9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84998-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01753-2

  Cytometric bead array standard curve of MP01753-2, ZNF618 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84998-1-PBS. Detection antibody: 84998-2-PBS. Standard: Ag36487. Range: 0.625-40 ng/mL.

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  Affinity and Kinetic Characterization of 84998-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84998-1-RR against Human ZNF618 were performed. The affinity constant is 1.64 nM.

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Geprüfte Anwendungen

Produktinformation

84998-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA ZNF618 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ZNF618 fusion protein Ag36487
• Vollständiger Name: zinc finger protein 618
• Berechnetes Molekulargewicht: 105kDa，954aa
• Beobachtetes Molekulargewicht: 110-130 kDa
• GenBank-Zugangsnummer: NM_001318042.1
• Gene symbol: ZNF618
• Gene ID (NCBI): 114991
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zinc finger protein 618 (ZNF618) is a key protein associated with DNA methylation and acts as a specific 5hmC reader in vivo to regulate UHRF2 function by promoting UHRF2 chromatin localization. In addition, ZNF618 is not only associated with the induction, maintenance or progression of female breast cancer, but is also up-regulated in lung cancer cells.