ZNF703 Polyklonaler Antikörper

ZNF703 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 21075-1-AP

Synonyme

ZPO1, ZNF503L, Zinc finger elbow-related proline domain protein 1, ZEPPO1

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  WB analysis of Jurkat using 21075-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21075-1-AP (ZNF703 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21075-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21075-1-AP (ZNF703 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 21075-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21075-1-AP (ZNF703 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 21075-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21075-1-AP (ZNF703 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 21075-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21075-1-AP (ZNF703 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse intestine using 21075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 21075-1-AP (ZNF703 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 21075-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 21075-1-AP (ZNF703 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 21075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21075-1-AP (ZNF703 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 21075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 21075-1-AP (ZNF703 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 21075-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 21075-1-AP (ZNF703 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 21075-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 21075-1-AP (ZNF703 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 21075-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 21075-1-AP (ZNF703 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, humanes Hirngewebe, Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes malignes Melanomgewebe, Maus-Cerebellum-Gewebe, Maushautgewebe, Ratten-Cerebellum-Gewebe, Rattenhautgewebe, Ratten-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

21075-1-AP bindet in WB, IHC, ELISA ZNF703 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZNF703 fusion protein Ag15242
• Vollständiger Name: zinc finger protein 703
• Berechnetes Molekulargewicht: 590 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC032534
• Gene symbol: ZNF703
• Gene ID (NCBI): 80139
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; Long noncoding RNA AFAP1-AS1 promotes tumor progression and invasion by regulating the miR-2110/Sp1 axis in triple-negative breast cancer.; Authors - Xiaohui Zhang