ZNF746 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ZNF746 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 24543-1-AP

Synonyme

Zinc finger protein 746, Parkin-interacting substrate, PARIS, FLJ31413

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  WB analysis using 24543-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24543-1-AP (ZNF746 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 24543-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 24543-1-AP (ZNF746 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HEK-293T-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

24543-1-AP bindet in WB, IHC, IF, ELISA ZNF746 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZNF746 fusion protein Ag21566
• Vollständiger Name: zinc finger protein 746
• Berechnetes Molekulargewicht: 644 aa, 69 kDa
• Beobachtetes Molekulargewicht: 85-95 kDa
• GenBank-Zugangsnummer: BC068505
• Gene symbol: ZNF746
• Gene ID (NCBI): 155061
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZNF746, also named as PARIS, which belongs to the krueppel C2H2-type zinc-finger protein family. ZNF746 mainly localizes to the cytoplasm and probably translocates to the nucleus to repress selected genes. ZNF746 as a transcription repressor that specifically binds to the 5'-TATTTT[T/G]-3' consensus sequence on promoters and repress transcription of PGC-1-alpha (PPARGC1A), thereby playing a role in regulation of neuron death. ZNF746 may act as a downstream effector of PRKN and contribute to neurodegeneration in Parkinson disease cases caused by defects in PRKN (PMID: 28122242). The calculated molecular weight of ZNF746 is 69 kDa, but Phosphorylation of ZNF746 is about 80-85 kDa (PMID: 28122242).

Publikationen

Species	Application	Title
mouse	WB	Neuron; Neuronal NLRP3 is a parkin substrate that drives neurodegeneration in Parkinson's disease.; Authors - Nikhil Panicker ; KO Validated
human,mouse	WB	Sci Adv; Deubiquitinase CYLD acts as a negative regulator of dopamine neuron survival in Parkinson's disease.; Authors - Sheila K Pirooznia
mouse	WB	Brain; Parkin interacting substrate zinc finger protein 746 is a pathological mediator in Parkinson's disease.; Authors - Saurav Brahmachari
human,mouse	WB,IF	Brain; Parkin interacting substrate phosphorylation by c-Abl drives dopaminergic neurodegeneration.; Authors - Hyojung Kim
mouse	WB	Front Aging Neurosci; Identification and evaluation of midbrain specific longevity-related genes in exceptionally long-lived but healthy mice; Authors - Hyojung Kim
human,mouse	WB,IHC	Biochem Biophys Res Commun; ZNF746/PARIS promotes the occurrence of hepatocellular carcinoma.; Authors - Hanna Kim ; KO Validated