ZNHIT1 Polyklonaler Antikörper

ZNHIT1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16595-1-PBS

Synonyme

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  WB analysis using 16595-1-AP (same clone as 16595-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16595-1-AP (ZNHIT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16595-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 16595-1-AP (same clone as 16595-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16595-1-AP (ZNHIT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16595-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 16595-1-AP (same clone as 16595-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16595-1-AP (ZNHIT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16595-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 16595-1-AP (same clone as 16595-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ZNHIT1 antibody (16595-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16595-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 16595-1-AP (same clone as 16595-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNHIT1 antibody (16595-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16595-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16595-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ZNHIT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZNHIT1 fusion protein Ag9741
• Vollständiger Name: zinc finger, HIT type 1
• Berechnetes Molekulargewicht: 154 aa, 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC017333
• Gene symbol: ZNHIT1
• Gene ID (NCBI): 10467
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZNHIT1(Zinc finger HIT domain containing protein 1), aslo named CGBP1 (Cyclin G1 binding protein 1), is a subunit of the SNF-2 related CBP activator protein (SRCAP) complex, which can remodel chromatin by incorporating the histone variant H2A.Z into nucleosomes(PMID: 15647280). ZNHIT1 also can bind to myogenin promoter and mediate H2A.Z incorporation for its expression(PMID: 20473270). ZNHIT1 overexpression induces apoptosis and represses proliferation and invasion of breast cancer cells(PMID: 30928405).