ZNRF2-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ZNRF2-Specific Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 20200-1-AP

Synonyme

Protein Ells2, RING finger protein 202, RNF202, zinc and ring finger 2, Zinc/RING finger protein 2, ZNRF2, ZNRF2-Specific

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  WB analysis using 20200-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20200-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20200-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 20200-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20200-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, Maushirngewebe, Mausnierengewebe, Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

20200-1-AP bindet in WB, ELISA ZNRF2-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: zinc and ring finger 2
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: NM_147128
• Gene symbol: ZNRF2
• Gene ID (NCBI): 223082
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZNRF2 also named as RNF202, may play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. The antibody is specific to ZNRF2.

Publikationen

Species	Application	Title
rat	WB	Exp Neurol; ZNRF2 attenuates focal cerebral ischemia/reperfusion injury in rats by inhibiting mTORC1-mediated autophagy.; Authors - Chao Gu
mouse	WB	Mol Neurobiol; Deletion of ZNRF2 Exacerbates MPTP-Induced Parkinson's Disease by Activating mTOR-Mediated Neuroinflammatory Pathways; Authors - Fanshi Zhang
human	WB	Hum Cell; ZNRF2 as an oncogene is transcriptionally regulated by CREB1 in breast cancer models; Authors - Jin-Tao Liu
human	WB	J Cell Mol Med; A pancancer analysis of the oncogenic role of ZNRF2 in human tumours; Authors - Fujie Shi ; KD Validated